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41.
Transfer of nitrogen from a tropical legume tree to an associated fodder grass via root exudation and common mycelial networks 总被引:1,自引:0,他引:1
Symbiotic dinitrogen fixation by legume trees represents a substantial N input in agroforestry systems, which may benefit the associated crops. Applying 15 N labelling, we studied N transfer via common mycelial networks (CMN) and root exudation from the legume tree Gliricidia sepium to the associated fodder grass Dichantium aristatum . The plants were grown in greenhouse in shared pots in full interaction (treatment FI) or with their root systems separated with a fine mesh that allowed N transfer via CMN only (treatment MY). Tree root exudation was measured separately with hydroponics. Nitrogen transfer estimates were based on the isotopic signature of N ( δ 15 N) transferred from the donor. We obtained a range for estimates by calculating transfer with δ 15 N of tree roots and exudates. Nitrogen transfer was 3.7–14.0 and 0.7–2.5% of grass total N in treatments FI and MY, respectively. Root δ 15 N gave the lower and exudate δ 15 N the higher estimates. Transfer in FI probably occurred mainly via root exudation. Transfer in MY correlated negatively with grass root N concentration, implying that it was driven by source-sink relationships between the plants. The range of transfer estimates, depending on source δ 15 N applied, indicates the need of understanding the transfer mechanisms as a basis for reliable estimates. 相似文献
42.
The potential of the nitrogen fixing bacteriumAzospirillum brasilense to enhance development and increase growth of several gramineae was investigated. In both sterilized and non-sterilized systems heading and flowering occurred earlier in the inoculated plants as compared to the noninoculated ones. Total shoot and root weights, total-N content, plant height and leaf length were significantly increased by inoculation.From the Department of Agricultural Botany. 相似文献
43.
Rice straw decomposition in rice-field soil 总被引:1,自引:0,他引:1
Rice straw, buried in a rice-field during the dry season decomposed at a rate of 0.0075 day-1. Seventy five percent of the biomass, 70 percent carbon, 50 percent nitrogen and 30 percent phosphorus remained after 139 days of decomposition. Rice straw decomposition furnished 33% N and 8% P of the total nitrogen and phosphorus provided by man. 相似文献
44.
M.J. Tisdale 《Chemico-biological interactions》1981,34(1):75-83
The tumour growth inhibitor L-2-amino-4-methoxy-trans-3-butenoic acid (Ro07-7957) inhibits serine hydroxymethyltransferase in cytosolic extracts of Walker carcinoma non-competitively with respect to L-serine with an apparent inhibition constant similar to the Km-value for L-serine. The kinetics of inactivation suggest that it reacts as an irreversible substrate analogue. Incubation of Walker cells with Ro07-7957 causes an increase in serine hydroxymethyltransferase activity which is most pronounced at concentration ≤LD50. This increase in enzyme activity does not occur in the presence of cycloheximide. These results suggest that inhibition of serine hydroxymethyltransferase in intact cells is accompanied by an increase in enzyme biosynthesis and that the growth inhibitory property of Ro07-7957 does not involve interference with the conversion of serine to glycine. 相似文献
45.
Summary The influence of total nitrification to nitrate or partial nitrification to nitrite on the soil organic nitrogen status was examined. NH
4
+
–15N was added to the soil in the absence and the presence of NaClO3, respectively nitrapyrin. The first chemical inhibits only nitrate formation, the second inhibits total nitrification. The accumulation of nitrite nitrogen in the soil at levels up to 5 mg kg–1 increased the loss of nitrogen. Yet, it did not increase the binding of mineral nitrogen into soil organic matter, relative to the control soil. The data suggest that the biochemistry of the nitrite formation process, rather than the levels of nitrite ions formed, are of primary importance in the role of nitrification mediated nitrosation of soil organic matter. 相似文献
46.
47.
Summary Growth responses of Frankia isolates to decreasing water potential were monitored in systems where potentials were controlled
by KCl, NaCl and Polyethylene glycol. The highest potential tested was −2 bar (basal medium). The general pattern emerging
was that isolates fromAlnus glutinosa, A. viridis andComptonia peregrina showed declining growth at potentials below −2 to −5 bar. AMyrica gale isolate showed declining growth with decreasing potential. All isolates were more sensitive to decreases in potential in
a matric controlled than an osmotic controlled system. They all showed approximately 50 percent growth reduction at −5 to
−8 bar, and meagre growth at −16 bar after 35 days. The Comptonia isolate was the most vigorous at low potentials. Nitrogen
fixation ability was monitored for two isolates. Highest specific activities were observed between −3 and −5 bar for the Myrica
isolate and between −5 and −7.5 bar for theA. glutinosa isolate. 相似文献
48.
The effect of different carbon and nitrogen sources on the production of toxin by Clostridium argentinense was examined. The toxin production by C. argentinense in coculture with Pseudomonas mendocina increased in all the cases in relation to that produced by monocultures independent of the nature of the source. Using dextrin as carbon source C. argentinense produced the highest levels of toxin both in monocultures (300 LD50/mL) and in cocultures with P. mendocina (5000 LD50/mL). Experiments run in a microfermenter showed that the slow growth of cocultures associated with the assimilation of dextrin and the pH and Eh profiles favoured the production of toxin. Of the nitrogen sources assayed, corn steep liquor sustained the highest levels of toxin in both monocultures and cocultures with 3 and 2.8 fold increases with respect to that obtained using proteose peptone. The toxin production by C. argentinense cultures and C. argentinense–P. mendocina cocultures was highly dependent on the nature of the carbon and nitrogen sources used in the culture media. Growth of C. argentinense on substrates slowly assimilated stimulated the production of toxin. 相似文献
49.
50.
State of the art and research needs for the impact category eutrophication are discussed. Eutrophication is a difficult impact
category because it includes emissions to both air and water — both subject to different environmental mechanisms — as well
as impacts occurring in different types of terrestrial and aquatic ecosystems. The possible fate processes are complex and
include transportation between different ecosystems. In some recent approaches, transportation modelling of air emissions
has been included. However, in general, the used characterisation methods do not integrate fate modelling, which is a limitation.
The definition of the impact indicator needs further research, too. The inclusion of other nutrients than those typically
considered should also be investigated. 相似文献